Journal: Frontiers in Molecular Biosciences

Article Title: Contribution of Increased Expression of Yin Yang 2 to Development of Cardiomyopathy

doi: 10.3389/fmolb.2020.00035

Figure Lengend Snippet: Impairedcell proliferation in double transgenic (dTg) mouse hearts. (A) Right panel: immunofluorescence staining was performed with sections from E14.5 control and dTg mouse embryos by using anti-pH3 (pH3: green, a cell proliferation marker) and anti-cTnT-AF647 (cTnT: red, a cardiomyocyte marker). DAPI (blue) was used for staining nuclei. Arrows indicate pH3+/cTnT+cardiomyocytes. Arrowheads indicateexamples of stained red blood cells. The left panel shows the statistical analysis of the data shown in the right panel of (A) . Cont, control. Four randomly selected fields from each sample were scored for pH3 + /cTnT + cardiomyocytes ( n = 3 per group). (B,C) Microarray analysis was performed with RNA purified from E14.5 control and dTg mouse embryos. The 10 most upregulated (B) and 10 most downregulated GO terms (C) are shown. (D) A heat map showing the dysregulated genes involved in cell cycle progression.

Article Snippet: Microarray service was provided by Phalanx Biotech (OneArray Express, San Diego, CA, United States).

Techniques: Transgenic Assay, Immunofluorescence, Staining, Control, Marker, Microarray, Purification

Journal: International Journal of Molecular Sciences

Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome

doi: 10.3390/ijms21093033

Figure Lengend Snippet: Expression levels of ( A ) BDH2 and ( B ) LCN2 mRNA in BM in MDS and control patients, including de novo CN-AML and normal BM. The expression levels of the BDH2 and LCN2 genes were normalized to the internal control β-actin to obtain the relative threshold cycle (ΔC T ). BDH2, hydroxybutyrate dehydrogenase type 2; BM, bone marrow; CN-AML, cytogenetically normal acute myeloid leukemia; LCN2, lipocalin 2; MDS, myelodysplastic syndrome; RA, refractory anemia; RAEB, refractory anemia with excess blasts; RARS, refractory anemia with ringed sideroblasts.

Article Snippet: In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells.

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome

doi: 10.3390/ijms21093033

Figure Lengend Snippet: RNA microarray analysis of BDH2 targeting genes. Group 1: genes related to mitochondria; group 2: genes that have been reported as oncogenes or tumor suppressor genes; group 3: genes that function to control the survival, growth, differentiation, proliferation, and effector function of tissues and cells. All of those genes showed statistical significance with p < 0.05.

Article Snippet: In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells.

Techniques: Microarray, Control

Journal:

Article Title: Epithelial cell-targeted transgene expression enables isolation of cyan fluorescent protein (CFP)-expressing prostate stem/progenitor cells

doi: 10.1007/s11248-010-9478-2

Figure Lengend Snippet: Co-expression of K5 and K8 in prostate epithelium. a FACS profile of CFP and RFP fluorescing prostate cells from double transgenic K5/CFP + K18/RFP mice. The CFP−/RFP+ cells in the upper left, and the CFP+/RFP+ cells are the cell populations whose gene expression profiles were compared by microarray analysis. b Single cell RT-PCR analysis of prostate cells from double transgenic K5/CFP + K18/RFP mice. K5 amplicon is 346 bp, and K18 amplicon is 427 bp. c, d Sections of: c proximal region of ventral prostate lobe from adult FVB/NJ male (bar = 20 μm), and d urogenital sinus from FVB/NJ day 16 fetus, immunostained for both K5 and K8 (bar = 100 μm). Cells co-expressing K5 and K8 are yellow, some of which are indicted by the arrows in panel c. See Fig. S8 for images of separate fluorescent channels of urogenital sinus

Article Snippet: Samples were sent to Phalanx Biotech Group (CA) for amplification and OneArrayTM Microarray service, including single color labeling, three replications, hybridizations, scanning, signal extractions, and basic data analysis.

Techniques: Expressing, Transgenic Assay, Gene Expression, Microarray, Reverse Transcription Polymerase Chain Reaction, Amplification